Protein_Domain
Lov2

Part:BBa_J18927:Design

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)


phot1-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment used by Nakasone et al. is 475K - 578G. The core domain 449E - 586R has reportedly lower stability at RT (aggregation).

  • gene synthesis
  • codon optimized for E. coli


Source

literature

gene synthesis

References